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M9480822.TXT
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1994-09-05
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Document 0822
DOCN M9480822
TI Detection of JC virus DNA by PCR in brain tissue and cerebrospinal fluid
from HIV infected patients.
DT 9410
AU Caldarelli Stefano R; Omodeo Zorini E; Rivolta GE; Achilli G; Cattaneo
E; Vago L; Boldorini R; Ferrante P; Biology Lab., Fondazione Don C.
Gnocchi IRCCS, Milano, Italy.
SO Abstr Gen Meet Am Soc Microbiol. 1994;94:623 (abstract no. V-36). Unique
Identifier : AIDSLINE ASM94/94313123
AB The correct diagnosis of progressive multifocal leukoencephalopathy
(PML), a severe demyelinating disease caused from JC Virus, is of
particular relevance in AIDS patients. For these reasons we set a
Polymerase Chain Reaction (PCR) method to detect JCV DNA both in brain
tissues collected at autopsy from 17 cases and in cerebrospinal fluid
(CSF) and urine of 7 AIDS patients with different neurological symptoms,
including a suspected PML case. The genomic region chosen for PCR
belongs to the Large T antigen of JCV and contains a Bam HI site that is
present only in JCV but not in BKV genome. JCV DNA has been detected in
7 (58.3%) paraffin-embedded, in 2 (66.6%) formalin-fixed, in 2 (100%)
frozen tissues and in 2 (100%) frozen CSF. Regarding the 7
neurologically ill patients only one, presenting clinical and
radiological PML signs, was positive both in CSF and urine for JCV DNA.
Our results indicate that PCR is useful in the diagnosis of PML at
autopsy but mainly that it is efficient, specific and practical for the
correct diagnosis and follow-up of living AIDS patients.
DE Autopsy AIDS-Related Opportunistic Infections/CEREBROSPINAL FLUID/
*DIAGNOSIS/URINE Brain/*MICROBIOLOGY DNA,
Viral/*ANALYSIS/CEREBROSPINAL FLUID/URINE Follow-Up Studies Human
Papovaviridae Infections/COMPLICATIONS/*DIAGNOSIS Polymerase Chain
Reaction/*METHODS Polyomavirus hominis 2/*ISOLATION & PURIF
Sensitivity and Specificity Tumor Virus
Infections/COMPLICATIONS/*DIAGNOSIS MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).